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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis using 20149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis of K-562 using 20149-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis of Raji using 20149-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis of Jurkat using 20149-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using DDX51 Polyclonal antibody (20149-1-AP)

    IP experiment of K-562 using 20149-1-AP

    IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug.

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DDX51 Polyclonal antibody

DDX51 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20149-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA, IF

Synonyms

EC:3.6.4.13, DEAD box protein 51, ATP-dependent RNA helicase DDX51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, Raji cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

20149-1-AP targets DDX51 in WB, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX51 fusion protein Ag13691 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
Calculated molecular weight 666 aa, 72 kDa
Observed molecular weight 72-80 kDa
GenBank accession numberBC040185
Gene Symbol DDX51
Gene ID (NCBI) 317781
RRIDAB_10665947
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N8A6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).

Protocols

Product Specific Protocols
WB protocol for DDX51 antibody 20149-1-APDownload protocol
IP protocol for DDX51 antibody 20149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang
View Article