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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX52 Polyclonal antibody

DDX52 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16660-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX52, HUSSY-19, HUSSY19, EC:3.6.4.13, DEAD box protein 52

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX52 Polyclonal antibody (16660-1-AP)

    WB analysis using 16660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DDX52 Polyclonal antibody (16660-1-AP)

    WB analysis of Jurkat using 16660-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX52 Polyclonal antibody (16660-1-AP)

    IF Staining of HepG2 using 16660-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16660-1-AP (DDX52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16660-1-AP targets DDX52 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX52 fusion protein Ag10118 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 52
Calculated molecular weight 599 aa, 67 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC041785
Gene symbol DDX52
Gene ID (NCBI) 11056
RRIDAB_1851052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DDX52 antibody 16660-1-APDownload protocol
IF protocol for DDX52 antibody 16660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols