RIG-1/DDX58 Polyclonal antibody
RIG-1/DDX58 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 25068-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, NIH/3T3 cells, THP-1 cells |
Positive IP detected in | A431 cells |
Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
IP | See 2 publications below |
Product Information
25068-1-AP targets RIG-1/DDX58 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RIG-1/DDX58 fusion protein Ag18585 相同性解析による交差性が予測される生物種 |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
Calculated molecular weight | 925 aa, 106 kDa |
Observed molecular weight | 101~106 kDa |
GenBank accession number | BC132786 |
Gene symbol | RIG-1/DDX58 |
Gene ID (NCBI) | 23586 |
RRID | AB_2879881 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RIG-1/DDX58 antibody 25068-1-AP | Download protocol |
IHC protocol for RIG-1/DDX58 antibody 25068-1-AP | Download protocol |
IP protocol for RIG-1/DDX58 antibody 25068-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Med RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma. | ||
bioRxiv A tale of two tumors: differential, but detrimental, effects of glioblastoma extracellular vesicles (EVs) on normal human brain cells | ||
Proc Natl Acad Sci U S A IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus | ||
bioRxiv IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH RASHMI (Verified Customer) (11-27-2024) | Used for WB, Highly recommended
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