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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX60 Polyclonal antibody

DDX60 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30982-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX60 Polyclonal antibody (30982-1-AP)

    WB analysis using 30982-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30982-1-AP (DDX60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using DDX60 Polyclonal antibody (30982-1-AP)

    IHC staining of human stomach using 30982-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30982-1-AP (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using DDX60 Polyclonal antibody (30982-1-AP)

    IHC staining of human stomach using 30982-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30982-1-AP (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using DDX60 Polyclonal antibody (30982-1-AP)

    IF Staining of U-251 using 30982-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DDX60 antibody (30982-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA549 cells, Jurkat cells, U-251 cells, U-87 MG cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

30982-1-AP targets DDX60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX60 fusion protein Ag34406 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Calculated molecular weight 1712 aa, 198 kDa
Observed molecular weight170-200 kDa
GenBank accession numberBC038115
Gene symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

Protocols

Product Specific Protocols
WB protocol for DDX60 antibody 30982-1-APDownload protocol
IHC protocol for DDX60 antibody 30982-1-APDownload protocol
IF protocol for DDX60 antibody 30982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Inflamm Res

Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis

Authors - Jingjing Xu
View Article
humanWB

Front Cell Dev Biol

Genome-wide analysis reveals the MORC3-mediated repression of PD-L1 expression in head and neck cancer

Authors - Wenxuan Fu
View Article