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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX60 Recombinant antibody (82969-1-RR)

    WB analysis using 82969-1-RR (same clone as 82969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82969-1-RR (DDX60 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using DDX60 Recombinant antibody (82969-1-RR)

    IHC staining of human stomach using 82969-1-RR (same clone as 82969-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82969-1-RR (DDX60 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DDX60 Recombinant antibody (82969-1-RR)

    IF Staining of A431 using 82969-1-RR (same clone as 82969-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using DDX60 Recombinant antibody (82969-1-RR)

    FC experiment of A431 using 82969-1-RR (same clone as 82969-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.2 ug DDX60 Recombinant antibody (82969-1-RR, Clone:230293C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82969-1-RR

    Affinity and Kinetic Characterization of 82969-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82969-1-RR against Human DDX60 were performed. The affinity constant is 0.145 nM.

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DDX60 Recombinant antibody, PBS Only

DDX60 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82969-1-PBS
Clone No.230293C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82969-1-PBS targets DDX60 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX60 fusion protein Ag34406 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Calculated molecular weight 1712 aa, 198 kDa
Observed molecular weight170-200 kDa
GenBank accession numberBC038115
Gene Symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY21
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.