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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX60 Recombinant antibody, PBS Only

DDX60 Recombinant Antibody for IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230293G10

Cat no : 82969-4-PBS

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DDX60 Recombinant antibody (82969-4-RR)

    IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DDX60 Recombinant antibody (82969-4-RR)

    IHC staining of human lung cancer using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using DDX60 Recombinant antibody (82969-4-RR)

    FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82969-4-PBS targets DDX60 in IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX60 fusion protein Ag34406 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Calculated molecular weight 1712 aa, 198 kDa
Observed molecular weight 170-200 kDa
GenBank accession numberBC038115
Gene symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.