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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DEAF1 Polyclonal antibody (18323-1-AP)

    WB analysis using 18323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DEAF1 Polyclonal antibody (18323-1-AP)

    WB analysis using 18323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human liver cancer using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human kidney using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human kidney using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human liver cancer using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human placenta using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human placenta using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human testis using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human testis using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human skin using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human skin using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human brain using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human brain using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human spleen using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human spleen using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human lung using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using DEAF1 Polyclonal antibody (18323-1-AP)

    IHC staining of human lung using 18323-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using DEAF1 Polyclonal antibody (18323-1-AP)

    IF Staining of Jurkat using 18323-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DEAF1 Polyclonal antibody

DEAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18323-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ZMYND5, Zinc finger MYND domain-containing protein 5, Suppressin, SPN, NUDR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, BxPC-3 cells, Jurkat cells, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18323-1-AP targets DEAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEAF1 fusion protein Ag13173 相同性解析による交差性が予測される生物種
Full Name deformed epidermal autoregulatory factor 1 (Drosophila)
Calculated molecular weight 565 aa, 59 kDa
Observed molecular weight 60-63 kDa, 72 kDa
GenBank accession numberBC053322
Gene Symbol DEAF1
Gene ID (NCBI) 10522
RRIDAB_2261595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75398
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DEAF1 antibody 18323-1-APDownload protocol
IHC protocol for DEAF1 antibody 18323-1-APDownload protocol
IF protocol for DEAF1 antibody 18323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols