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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DEDD2 Polyclonal antibody

DEDD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14574-1-AP

Synonyms

DED containing protein FLAME 3, DEDD2, FLAME 3, FLAME3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using DEDD2 Polyclonal antibody (14574-1-AP)

    WB analysis of HEK-293 using 14574-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DEDD2 Polyclonal antibody (14574-1-AP)

    WB analysis of HEK-293 using 14574-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using DEDD2 Polyclonal antibody (14574-1-AP)

    IHC staining of human brain using 14574-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using DEDD2 Polyclonal antibody (14574-1-AP)

    IHC staining of human brain using 14574-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14574-1-AP targets DEDD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEDD2 fusion protein Ag5724 相同性解析による交差性が予測される生物種
Full Name death effector domain containing 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC027930
Gene symbol DEDD2
Gene ID (NCBI) 162989
RRIDAB_2091538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DEDD2 antibody 14574-1-APDownload protocol
IHC protocol for DEDD2 antibody 14574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Authors - Leah Fitzsimmons
View Article