Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human small intestine tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
18057-1-AP targets DEFA1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DEFA1 fusion protein Ag12592 相同性解析による交差性が予測される生物種 |
| Full Name | defensin, alpha 1 |
| Calculated molecular weight | 94 aa, 10 kDa |
| Observed molecular weight | 6 kDa |
| GenBank accession number | BC093791 |
| Gene Symbol | DEFA1 |
| Gene ID (NCBI) | 1667 |
| RRID | AB_2292842 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P59665 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DEFA1 antibody 18057-1-AP | Download protocol |
| IHC protocol for DEFA1 antibody 18057-1-AP | Download protocol |
| IF protocol for DEFA1 antibody 18057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1. | ||
Hum Cell Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer. | ||
Syst Biol Reprod Med Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts. | ||
Mediators Inflamm Potential Association of Gut Microbial Metabolism and Circulating mRNA Based on Multiomics Sequencing Analysis in Fetal Growth Restriction |