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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DEFA1 Monoclonal antibody, PBS Only

DEFA1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B3

Cat no : 67156-1-PBS

Synonyms

DEF1, DEFA1, DEFA1B, DEFA2, defensin, alpha 1, HNP 1, HNP 2, HP 1, HP 2, HP1, HP2, MRS, Neutrophil defensin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood leukocyte using DEFA1 Monoclonal antibody (67156-1-Ig)

    WB analysis of human peripheral blood leukocyte using 67156-1-Ig (same clone as 67156-1-PBS)

    Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 67156-1-Ig (DEFA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DEFA1 Monoclonal antibody (67156-1-Ig)

    IHC staining of human small intestine using 67156-1-Ig (same clone as 67156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DEFA1 Monoclonal antibody (67156-1-Ig)

    IHC staining of human small intestine using 67156-1-Ig (same clone as 67156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using DEFA1 Monoclonal antibody (67156-1-Ig)

    IF Staining of mouse spleen using 67156-1-Ig (same clone as 67156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using DEFA1 antibody (67156-1-Ig, Clone: 1A2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67156-1-PBS targets DEFA1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DEFA1 fusion protein Ag12592 相同性解析による交差性が予測される生物種
Full Name defensin, alpha 1
Calculated molecular weight 94 aa, 10 kDa
Observed molecular weight 6-10 kDa
GenBank accession numberBC093791
Gene symbol DEFA1
Gene ID (NCBI) 1667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.