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  • Featured Product
  • KD/KO Validated

DEK Monoclonal antibody, PBS Only

DEK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5E3A6

Cat no : 66194-1-PBS

Synonyms

D6S231E, 5E3A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DEK Monoclonal antibody (66194-1-Ig)

    WB analysis using 66194-1-Ig (same clone as 66194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66194-1-Ig (DEK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DEK Monoclonal antibody (66194-1-Ig)

    WB analysis of HEK-293 using 66194-1-Ig (same clone as 66194-1-PBS)

    WB result of DEK antibody (66194-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells. This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DEK Monoclonal antibody (66194-1-Ig)

    IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DEK Monoclonal antibody (66194-1-Ig)

    IHC staining of human placenta using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66194-1-Ig (DEK antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DEK Monoclonal antibody (66194-1-Ig)

    IF Staining of HepG2 using 66194-1-Ig (same clone as 66194-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DEK antibody (66194-1-Ig, Clone: 5E3A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66194-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66194-1-PBS targets DEK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DEK fusion protein Ag8225 相同性解析による交差性が予測される生物種
Full Name DEK oncogene
Calculated molecular weight 43 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC035259
Gene symbol DEK
Gene ID (NCBI) 7913
RRIDAB_2881588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.