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  • Featured Product
  • KD/KO Validated

DEK Recombinant antibody

DEK Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230065H2

Cat no : 82880-1-RR

Synonyms

D6S231E, 230065H2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DEK Recombinant antibody (82880-1-RR)

    WB analysis of HEK-293 using 82880-1-RR

    WB result of DEK antibody (82880-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using DEK Recombinant antibody (82880-1-RR)

    WB analysis using 82880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82880-1-RR (DEK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using DEK Recombinant antibody (82880-1-RR)

    IHC staining of human skin cancer using 82880-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82880-1-RR (DEK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DEK Recombinant antibody (82880-1-RR)

    IF Staining of U2OS using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DEK Recombinant antibody (82880-1-RR)

    IF Staining of HeLa using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Flow cytometry (FC) experiment of HeLa cells using DEK Recombinant antibody (82880-1-RR)

    FC experiment of HeLa using 82880-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DEK Recombinant antibody (82880-1-RR, Clone:230065H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82880-1-RR

    Affinity and Kinetic Characterization of 82880-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82880-1-RR against Human DEK were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82880-1-RR targets DEK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DEK fusion protein Ag8225 相同性解析による交差性が予測される生物種
Full Name DEK oncogene
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC035259
Gene symbol DEK
Gene ID (NCBI) 7913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Protocols

Product Specific Protocols
WB protocol for DEK antibody 82880-1-RRDownload protocol
IHC protocol for DEK antibody 82880-1-RRDownload protocol
IF protocol for DEK antibody 82880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols