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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DENND3 Polyclonal antibody

DENND3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24414-1-AP

Synonyms

DENN/MADD domain containing 3, DENND3, KIAA0870

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse stomach tissue using DENND3 Polyclonal antibody (24414-1-AP)

    WB analysis of mouse stomach using 24414-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse stomach tissue using DENND3 Polyclonal antibody (24414-1-AP)

    WB analysis of mouse stomach using 24414-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24414-1-AP is DENND3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DENND3 fusion protein Ag19859 相同性解析による交差性が予測される生物種
Full Name DENN/MADD domain containing 3
Calculated molecular weight 1198 aa, 135 kDa
Observed molecular weight 135-145 kDa
GenBank accession numberBC133023
Gene symbol DENND3
Gene ID (NCBI) 22898
RRIDAB_2879533
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DENND3 may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor

Protocols

Product Specific Protocols
WB protocol for DENND3 antibody 24414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

Identification of lysosome-related hub genes as potential biomarkers and immune infiltrations of moyamoya disease by multiple bioinformatics methods and machine-learning strategies

Authors - Wenyang Li
View Article