Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | DEP domain containing 6 |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | NM_022783 |
| Gene Symbol | DEPDC6 |
| Gene ID (NCBI) | 64798 |
| RRID | AB_11182391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TB45 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DEPDC6/Deptor antibody 20985-1-AP | Download protocol |
| IHC protocol for DEPDC6/Deptor antibody 20985-1-AP | Download protocol |
| IF protocol for DEPDC6/Deptor antibody 20985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling. | ||
Autophagy TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes. | ||
Cell Death Differ NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex. | ||
Oncotarget Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation. | ||
Stem Cell Res Ther DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis. | ||