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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DEPDC6 Polyclonal antibody, PBS Only

DEPDC6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21312-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of HeLa using 21312-1-AP (same clone as 21312-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of HT-1080 using 21312-1-AP (same clone as 21312-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of COLO 320 using 21312-1-AP (same clone as 21312-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DEPDC6 Polyclonal antibody (21312-1-AP)

    IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DEPDC6 Polyclonal antibody (21312-1-AP)

    IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21312-1-PBS targets DEPDC6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEPDC6 fusion protein Ag15959 相同性解析による交差性が予測される生物種
Full Name DEP domain containing 6
Calculated molecular weight 409 aa, 46 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC024746
Gene symbol DEPDC6
Gene ID (NCBI) 64798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.