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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DEPDC7 Polyclonal antibody (26517-1-AP)

    WB analysis of HepG2 using 26517-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DEPDC7 Polyclonal antibody (26517-1-AP)

    WB analysis of HeLa using 26517-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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DEPDC7 Polyclonal antibody

DEPDC7 Polyclonal Antibody for WB, ELISA
Cat No. 26517-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TR2, Protein TR2/D15, DEP domain-containing protein 7, DEP domain containing 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26517-1-AP targets DEPDC7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEPDC7 fusion protein Ag24094 相同性解析による交差性が予測される生物種
Full Name DEP domain containing 7
Observed molecular weight 55-60 kDa
GenBank accession numberBC030970
Gene Symbol DEPDC7
Gene ID (NCBI) 91614
RRIDAB_2880539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEPDC7 (DEP domain-containing protein 7), also known as protein TR2/D15, is a 511 amino acid protein belonging to the DEPDC7 family. DEPDC7 contains one DEP domain and is highly expressed in liver, with lower levels in kidney. DEPDC7 exists as two isoforms produced by alternative splicing events. The gene encoding DEPDC7 maps to human chromosome 11p13 and mouse chromosome 2 E2. With approximately 135 million base pairs and 1,400 genes, chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. DEPDC7 involves the regulation of small GTPase mediated signal transduction.

Protocols

Product Specific Protocols
WB protocol for DEPDC7 antibody 26517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols