DFFA,DFF45 Monoclonal antibody, PBS Only
DFFA,DFF45 Monoclonal Antibody for WB, IF, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, IF, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
1F6H9
Cat no : 68177-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
68177-1-PBS targets DFFA,DFF45 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | DFFA,DFF45 fusion protein Ag32657 相同性解析による交差性が予測される生物種 |
Full Name | DNA fragmentation factor, 45kDa, alpha polypeptide |
Calculated molecular weight | 37 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC000037 |
Gene symbol | DFFA |
Gene ID (NCBI) | 1676 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.