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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DFNA5 Polyclonal antibody, PBS Only

DFNA5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31363-1-PBS

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DFNA5 Polyclonal antibody (31363-1-AP)

    WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DFNA5 Polyclonal antibody (31363-1-AP)

    WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using DFNA5 Polyclonal antibody (31363-1-AP)

    WB analysis of mouse brain using 31363-1-AP (same clone as 31363-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using DFNA5 Polyclonal antibody (31363-1-AP)

    IP experiment of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DFNA5 Polyclonal antibody (31363-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DFNA5 Polyclonal antibody (31363-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31363-1-PBS targets DFNA5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DFNA5 fusion protein Ag35186 相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight55 kDa, 35 kDa, 25 kDa
GenBank accession numberBC019689
Gene symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.