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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DFNA5 Recombinant antibody, PBS Only (Capture)

DFNA5 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240394B3

Cat no : 83454-1-PBS

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00455-2

    Sandwich ELISA standard curve of MP00455-2

    Sandwich ELISA standard curve of MP00455-2, Human DFNA5 Recombinant Matched Antibody Pair - PBS only. 83454-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3746. 83454-4-PBS was HRP conjugated as the detection antibody. Range: 1.88-120 ng/mL

  • Sample test of MP00455-2

    Sample test of MP00455-2

    HCT116 cell lysate was measured. The human DFNA5 concentration of detected samples was determined to be 5.90 µg/mL (based on a 4.3 mg/mL extract load).

  • Cytometric bead array standard curve of MP00455-2

    Cytometric bead array standard curve of MP00455-2

    Cytometric bead array standard curve of MP00455-2, DFNA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83454-1-PBS. Detection antibody: 83454-4-PBS. Standard: Ag3746. Range: 0.78-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83454-1-PBS targets DFNA5 as part of a matched antibody pair:

MP00455-2: 83454-1-PBS capture and 83454-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DFNA5 fusion protein Ag3746 相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
GenBank accession numberBC019689
Gene symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.