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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DFNA5 Recombinant antibody

DFNA5 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240394D2

Cat no : 83454-6-RR

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DFNA5 Recombinant antibody (83454-6-RR)

    WB analysis using 83454-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83454-6-RR

    Affinity and Kinetic Characterization of 83454-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83454-6-RR against Human DFNA5 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83454-6-RR is DFNA5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DFNA5 fusion protein Ag3746 相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC019689
Gene symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

Protocols

Product Specific Protocols
WB protocol for DFNA5 antibody 83454-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols