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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DFNA5 Polyclonal antibody

DFNA5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 31363-1-AP

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Polyclonal antibody (31363-1-AP)

    WB analysis using 31363-1-AP

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Polyclonal antibody (31363-1-AP)

    WB analysis using 31363-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DFNA5/GSDME Polyclonal antibody (31363-1-AP)

    WB analysis of mouse brain using 31363-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using DFNA5/GSDME Polyclonal antibody (31363-1-AP)

    IP experiment of SH-SY5Y using 31363-1-AP

    IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DFNA5 Polyclonal antibody (31363-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DFNA5 Polyclonal antibody (31363-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, A549 cells, mouse brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31363-1-AP targets DFNA5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DFNA5 fusion protein Ag35186 相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight55 kDa, 35 kDa, 25 kDa
GenBank accession numberBC019689
Gene symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

Protocols

Product Specific Protocols
WB protocol for DFNA5 antibody 31363-1-APDownload protocol
IHC protocol for DFNA5 antibody 31363-1-APDownload protocol
IP protocol for DFNA5 antibody 31363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols