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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Monoclonal antibody (67731-1-Ig)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Monoclonal antibody (67731-1-Ig)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using DFNA5/GSDME Monoclonal antibody (67731-1-Ig)

    FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human DFNA5/GSDME (67731-1-Ig, Clone:1D9C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

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DFNA5/GSDME Monoclonal antibody, PBS Only

DFNA5/GSDME Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 67731-1-PBS
Clone No.1D9C3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67731-1-PBS targets DFNA5/GSDME in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DFNA5/GSDME fusion protein Ag30514 相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
RRIDAB_2882917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60443
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.