• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DFNA5 Monoclonal antibody, PBS Only

DFNA5 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9C3

Cat no : 67731-1-PBS

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DFNA5 Monoclonal antibody (67731-1-Ig)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DFNA5 Monoclonal antibody (67731-1-Ig)

    WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using DFNA5 Monoclonal antibody (67731-1-Ig)

    FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human DFNA5/GSDME (67731-1-Ig, Clone:1D9C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67731-1-PBS targets DFNA5 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DFNA5 fusion protein Ag30514 相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC019689
Gene symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.