Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, mouse testis tissue, Jurkat cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10996-1-AP targets DGCR8 C-terminal in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DGCR8 C-terminal fusion protein Ag1429 相同性解析による交差性が予測される生物種 |
| Full Name | DiGeorge syndrome critical region gene 8 |
| Calculated molecular weight | 773 aa, 86 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC009323 |
| Gene Symbol | DGCR8 |
| Gene ID (NCBI) | 54487 |
| RRID | AB_2090987 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WYQ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| IHC protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| IF protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| IP protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer. | ||
Cell Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis. | ||
Cell Stem Cell Principles of signaling pathway modulation for enhancing human naive pluripotency induction. |