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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    WB analysis of A431 cells using 25835-1-AP

    WB result of DGCR8 antibody (25835-1-AP, 1:2000) with si-control and si-DGCR8 transfected A431 cells.

  • Western Blot (WB) analysis of HeLa cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    WB analysis of HeLa using 25835-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    WB analysis of HEK-293 using 25835-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    WB analysis of Jurkat using 25835-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    WB analysis of HepG2 using 25835-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    WB analysis of A431 using 25835-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    IP experiment of A431 using 25835-1-AP

    IP result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    IHC staining of human ovary cancer using 25835-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DGCR8 N-terminal Polyclonal antibody (25835-1-AP)

    IF Staining of HeLa using 25835-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

DGCR8 N-terminal Polyclonal antibody

DGCR8 N-terminal Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25835-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DGCR8, C22orf12, pasha, LP4941, Gy1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

25835-1-AP targets DGCR8 N-terminal in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGCR8 N-terminal fusion protein Ag22988 相同性解析による交差性が予測される生物種
Full Name DiGeorge syndrome critical region gene 8
Calculated molecular weight 773 aa, 86 kDa
Observed molecular weight 88-100 kDa
GenBank accession numberBC078147
Gene Symbol DGCR8
Gene ID (NCBI) 54487
RRIDAB_2880262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Protocols

Product Specific Protocols
WB protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
IHC protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
IF protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
IP protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nucleic Acids Res

DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity

Authors - An Yan
View Article
bovineIF

Proc Natl Acad Sci U S A

Embryonic microRNAs are essential for bovine preimplantation embryo development

Authors - Erika E Paulson
View Article