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  • Featured Product
  • KD/KO Validated

DGCR8 Monoclonal antibody, PBS Only

DGCR8 Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D11A3

Cat no : 60084-1-PBS

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DGCR8 Monoclonal antibody (60084-1-Ig)

    WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using DGCR8 Monoclonal antibody (60084-1-Ig)

    WB analysis of RAW 264.7 using 60084-1-Ig (same clone as 60084-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DGCR8 Monoclonal antibody (60084-1-Ig)

    WB analysis of A431 using 60084-1-Ig (same clone as 60084-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DGCR8 Monoclonal antibody (60084-1-Ig)

    WB analysis of HepG2 using 60084-1-Ig (same clone as 60084-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using DGCR8 Monoclonal antibody (60084-1-Ig)

    WB analysis of BxPC-3 using 60084-1-Ig (same clone as 60084-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using DGCR8 Monoclonal antibody (60084-1-Ig)

    IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

    IP result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60084-1-PBS targets DGCR8 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DGCR8 fusion protein Ag4871 相同性解析による交差性が予測される生物種
Full Name DiGeorge syndrome critical region gene 8
Calculated molecular weight 773 aa, 86 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC009323
Gene symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.