Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
13873-1-AP targets DGKH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DGKH fusion protein Ag4926 相同性解析による交差性が予測される生物種 |
| Full Name | diacylglycerol kinase, eta |
| Calculated molecular weight | 1100 aa, 122 kDa |
| Observed molecular weight | 135 kDa |
| GenBank accession number | BC043292 |
| Gene Symbol | DGKH |
| Gene ID (NCBI) | 160851 |
| RRID | AB_2091287 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86XP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DGKH(Diacylglycerol kinase eta) is also named as DGK-eta and belongs to the eukaryotic diacylglycerol kinase family. It is a key protein in the lithium-sensitive phosphatidyl inositol pathway, and recently a link between environmental stress and schizophrenia/bipolar disorder has been revealed. DGKH has some isoforms produced by alternative splicing. Two isoforms DGKH1 and DGKH2 with the molecular weight of 128 kDa and 135 kDa shows diacylglycerol kinase activity; however, the 2 isoforms differed in several other characteristics(PMID:12810723).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DGKH antibody 13873-1-AP | Download protocol |
| IHC protocol for DGKH antibody 13873-1-AP | Download protocol |
| IF protocol for DGKH antibody 13873-1-AP | Download protocol |
| IP protocol for DGKH antibody 13873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes. | ||
BMC Dev Biol Distinct expression and localization of the type II diacylglycerol kinase isozymes ¿, ¿ and ¿ in the mouse reproductive organs. | ||
J Histochem Cytochem Expression and Localization of Type II Diacylglycerol Kinase Isozymes δ and η in the Developing Mouse Brain. | ||
Clin Transl Oncol Diacylglycerol kinase η modulates oncogenic properties of lung cancer cells.
| ||
Biochim Biophys Acta Mol Cell Biol Lipids Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells. | ||
Cancer Med Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma |