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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DGKZ Polyclonal antibody (32124-1-AP)

    WB analysis using 32124-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32124-1-AP (DGKZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DGKZ Polyclonal antibody (32124-1-AP)

    IF Staining of A431 using 32124-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKZ antibody (32124-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DGKZ Polyclonal antibody

DGKZ Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32124-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Diacylglycerol kinase zeta, DGK-zeta, DGK ZETA, DAGK6, DAGK5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32124-1-AP targets DGKZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKZ fusion protein Ag37336 相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, zeta 104kDa
Calculated molecular weight104kDa，928aa
Observed molecular weight 104-124 kDa
GenBank accession numberNM_001199267.1
Gene Symbol DGKZ
Gene ID (NCBI) 8525
RRIDAB_3670200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ13574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Diacylglycerol kinase zeta (DGKZ), also known as DAGK5, DAGK6, DGK-ZETA, hDGKzeta, belongs to the diacylglycerol kinase (DGK) family which catalyzes diacylglycerol (DAG) into phosphatidic acid (PA). DGKZ is a novel promoter of metastasis and that it could be a potential prognostic indicator in patients with triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFbetaR2(PMID: 35115500). Downregulation of DGKZ suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest(PMID: 33926342).

Protocols

Product Specific Protocols
WB protocol for DGKZ antibody 32124-1-APDownload protocol
IF protocol for DGKZ antibody 32124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols