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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHCR24 Monoclonal antibody (60028-1-Ig)

    WB analysis using 60028-1-Ig (same clone as 60028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DHCR24 Monoclonal antibody (60028-1-Ig)

    WB analysis of HepG2 using 60028-1-Ig (same clone as 60028-1-PBS)

    WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DHCR24 Monoclonal antibody (60028-1-Ig)

    IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DHCR24 Monoclonal antibody (60028-1-Ig)

    IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51020-1

    Cytometric bead array standard curve of MP51020-1

    Cytometric bead array standard curve of MP51020-1, DHCR24 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60028-2-PBS. Detection antibody: 60028-1-PBS. Standard:Ag0756. Range: 0.098-100 ng/mL.

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DHCR24 Monoclonal antibody, PBS Only (Detector)

DHCR24 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 60028-1-PBS
Clone No.2F6E11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

DHC24, EC:1.3.1.72, Delta(24)-sterol reductase, 3-beta-hydroxysterol Delta-24-reductase, 2F6E11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60028-1-PBS targets DHCR24 as part of a matched antibody pair:

MP51020-1: 60028-2-PBS capture and 60028-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHCR24 fusion protein Ag0756 相同性解析による交差性が予測される生物種
Full Name 24-dehydrocholesterol reductase
Calculated molecular weight 60 kDa, 516 aa
Observed molecular weight 60 kDa
GenBank accession numberBC004375
Gene Symbol DHCR24
Gene ID (NCBI) 1718
RRIDAB_874115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15392
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).