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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis using 15194-1-AP (same clone as 15194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    WB result of DHFR antibody (15194-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of Jurkat using 15194-1-AP (same clone as 15194-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of Raji using 15194-1-AP (same clone as 15194-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of K-562 using 15194-1-AP (same clone as 15194-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using DHFR Polyclonal antibody (15194-1-AP)

    IP experiment of Raji using 15194-1-AP (same clone as 15194-1-PBS)

    IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHFR Polyclonal antibody (15194-1-AP)

    IF Staining of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DHFR Polyclonal antibody, PBS Only

DHFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15194-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15194-1-PBS targets DHFR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHFR fusion protein Ag7331 相同性解析による交差性が予測される生物種
Full Name dihydrofolate reductase
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
RRIDAB_2091587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00374
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).