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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHFR Recombinant antibody (81021-1-RR)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHFR Recombinant antibody (81021-1-RR)

    WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DHFR Recombinant antibody (81021-1-RR)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Recombinant antibody (81021-1-RR)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Recombinant antibody (81021-1-RR)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81021-1-RR

    Affinity and Kinetic Characterization of 81021-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

DHFR Recombinant antibody, PBS Only

DHFR Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81021-1-PBS
Clone No.7P5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:1.5.1.3, DHFRP1, 7P5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81021-1-PBS targets DHFR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DHFR fusion protein Ag7331 相同性解析による交差性が予測される生物種
Full Name dihydrofolate reductase
Calculated molecular weight 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00374
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).