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  • KD/KO Validated

DHODH Polyclonal antibody

DHODH Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA
Cat No. 14877-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 500 ug/ml
20 μL, 500 ug/ml
150 μL, 500 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA2780 cells, MCF-7 cells, SKOV-3 cells, mouse heart tissue, mouse ovary tissue, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14877-1-AP targets DHODH in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHODH fusion protein Ag6649 相同性解析による交差性が予測される生物種
Full Name dihydroorotate dehydrogenase
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC065245
Gene Symbol DHODH
Gene ID (NCBI) 1723
RRIDAB_2091723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Protocols

Product Specific Protocols
WB protocol for DHODH antibody 14877-1-APDownload protocol
IHC protocol for DHODH antibody 14877-1-APDownload protocol
IF protocol for DHODH antibody 14877-1-APDownload protocol
IP protocol for DHODH antibody 14877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
humanWB

Nature

DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.

Authors - Chao Mao
  • KO Validated
human,mouseWB

Science

Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.

Authors - Issam Ben-Sahra
human,mouseWB

Cancer Discov

PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition.

Authors - Deepti Mathur
mouseIHC

Cancer Cell

Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma

Authors - William D Gwynne
  • KO Validated
mouseWB

Cell Metab

Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.

Authors - Christopher J Halbrook
  • KD Validated
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