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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHPS Polyclonal antibody

DHPS Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11184-1-AP

Synonyms

MIG13, EC:2.5.1.46, DS, DHS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DHPS Polyclonal antibody (11184-1-AP)

    WB analysis of HeLa using 11184-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11184-1-AP (DHPS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using DHPS Polyclonal antibody (11184-1-AP)

    IP experiment of NIH/3T3 using 11184-1-AP

    IP result of anti-DHPS (IP:11184-1-AP, 4ug; Detection:11184-1-AP 1:300) with NIH/3T3 cells lysate 3200 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHPS Polyclonal antibody (11184-1-AP)

    IF Staining of HeLa using 11184-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DHPS antibody (11184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

11184-1-AP targets DHPS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHPS fusion protein Ag1646 相同性解析による交差性が予測される生物種
Full Name deoxyhypusine synthase
Calculated molecular weight 41 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014016
Gene symbol DHPS
Gene ID (NCBI) 1725
RRIDAB_2877751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DHPS antibody 11184-1-APDownload protocol
IF protocol for DHPS antibody 11184-1-APDownload protocol
IP protocol for DHPS antibody 11184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

BMC Med

Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke.

Authors - Takaki Hiwasa
View Article