Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11184-1-AP targets DHPS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DHPS fusion protein Ag1646 相同性解析による交差性が予測される生物種 |
| Full Name | deoxyhypusine synthase |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC014016 |
| Gene Symbol | DHPS |
| Gene ID (NCBI) | 1725 |
| RRID | AB_2877751 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49366 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DHPS antibody 11184-1-AP | Download protocol |
| IF protocol for DHPS antibody 11184-1-AP | Download protocol |
| IP protocol for DHPS antibody 11184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Med Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke. | ||
Heliyon Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer |