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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis using 67998-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis of LNCaP using 67998-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis of HeLa using 67998-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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DHPS Monoclonal antibody

DHPS Monoclonal Antibody for WB, ELISA
Cat No. 67998-1-Ig
Clone No.1E4F5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

deoxyhypusine synthase, DHPS, DHS, DS, MIG13

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67998-1-Ig targets DHPS in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHPS fusion protein Ag31046 相同性解析による交差性が予測される生物種
Full Name deoxyhypusine synthase
Calculated molecular weight 41 kDa
GenBank accession numberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
RRIDAB_2918746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49366
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DHPS antibody 67998-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols