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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using DHRS2 Polyclonal antibody (15735-1-AP)

    WB analysis of SKOV-3 using 15735-1-AP (same clone as 15735-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using DHRS2 Polyclonal antibody (15735-1-AP)

    WB analysis of human liver using 15735-1-AP (same clone as 15735-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DHRS2 Polyclonal antibody (15735-1-AP)

    WB analysis using 15735-1-AP (same clone as 15735-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DHRS2 Polyclonal antibody (15735-1-AP)

    IP experiment of HeLa using 15735-1-AP (same clone as 15735-1-PBS)

    IP result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHRS2 Polyclonal antibody (15735-1-AP)

    IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHRS2 Polyclonal antibody (15735-1-AP)

    IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DHRS2 Polyclonal antibody, PBS Only

DHRS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15735-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15735-1-PBS targets DHRS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS2 fusion protein Ag8508 相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 2
Calculated molecular weight 300 aa, 32 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC007339
Gene Symbol DHRS2
Gene ID (NCBI) 10202
RRIDAB_2091847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13268
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.