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KD/KO Validated

DHRS3 Polyclonal antibody

Name: DHRS3 Polyclonal antibody
Brand: Proteintech
SKU: 15393-1-AP

DHRS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15393-1-AP

Synonyms

retSDR1, Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, RDH17, EC:1.1.1.300

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis using 15393-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 15393-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 15393-1-AP
IP result of anti-DHRS3 (IP:15393-1-AP, 4ug; Detection:15393-1-AP 1:500) with A375 cells lysate 3600ug.
IHC staining of mouse liver using 15393-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 15393-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15393-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS3 antibody (15393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15393-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue
Positive IP detected in	A375 cells
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 16 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

15393-1-AP targets DHRS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, insect
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DHRS3 fusion protein Ag7589 相同性解析による交差性が予測される生物種
Full Name	dehydrogenase/reductase (SDR family) member 3
Calculated molecular weight	34 kDa
Observed molecular weight	30-34 kDa
GenBank accession number	BC002730
Gene symbol	DHRS3
Gene ID (NCBI)	9249
RRID	AB_2091855
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).

Protocols

Product Specific Protocols
WB protocol for DHRS3 antibody 15393-1-AP	Download protocol
IHC protocol for DHRS3 antibody 15393-1-AP	Download protocol
IF protocol for DHRS3 antibody 15393-1-AP	Download protocol
IP protocol for DHRS3 antibody 15393-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Endocrinol Metab Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells. Authors - Kuroda Keiji K View Article
mouse	IHC	FASEB J The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development. Authors - Billings Sara E SE View Article
human,mouse	WB	J Biol Chem Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice. Authors - Olga V Belyaeva KD Validated View Article
mouse	WB	FASEB J Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage. Authors - Junmei Zhou View Article
human	WB	Mol Endocrinol Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells. Authors - Kuroda Keiji K View Article
human	WB,IF	J Biol Chem Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture. Authors - Lee Seung-Ah SA View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHRS3 Polyclonal antibody

DHRS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15393-1-AP

WB analysis using 15393-1-AP

IP experiment of A375 using 15393-1-AP

IHC staining of mouse liver using 15393-1-AP

IHC staining of mouse liver using 15393-1-AP

IF Staining of HepG2 using 15393-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells.

The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development.

Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice.

Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage.

Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells.

Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture.