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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHRS4 Polyclonal antibody

DHRS4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15279-1-AP

Synonyms

CR, DHRS4, DHRS4L2, humNRDR, NRDR, PHCR, PSCD, SCAD SRL, SDR SRL, SDR25C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis using 15279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of rat liver using 15279-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of HepG2 using 15279-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse liver tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of mouse liver using 15279-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of HeLa using 15279-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IP experiment of mouse liver using 15279-1-AP

    IP result of anti-DHRS4 (IP:15279-1-AP, 4ug; Detection:15279-1-AP 1:400) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervical cancer using 15279-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervical cancer using 15279-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervix tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervix using 15279-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervix using 15279-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inrat liver tissue, mouse liver tissue, HeLa cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman cervical cancer tissue, human cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15279-1-AP targets DHRS4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS4 fusion protein Ag7398 相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 4
Calculated molecular weight 30 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC003019
Gene symbol DHRS4
Gene ID (NCBI) 10901
RRIDAB_2292856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).

Protocols

Product Specific Protocols
WB protocol for DHRS4 antibody 15279-1-APDownload protocol
IHC protocol for DHRS4 antibody 15279-1-APDownload protocol
IP protocol for DHRS4 antibody 15279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Identification of Molecular Correlations Between DHRS4 and Progressive Neurodegeneration in Amyotrophic Lateral Sclerosis By Gene Co-Expression Network Analysis.

Authors - Shu Li
View Article