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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS7 Polyclonal antibody (15061-1-AP)

    WB analysis using 15061-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 15061-1-AP (DHRS7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using DHRS7 Polyclonal antibody (15061-1-AP)

    IHC staining of rat skeletal muscle using 15061-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15061-1-AP (DHRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DHRS7 Polyclonal antibody (15061-1-AP)

    IHC staining of mouse skeletal muscle using 15061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15061-1-AP (DHRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DHRS7 Polyclonal antibody (15061-1-AP)

    IF Staining of HepG2 using 15061-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS7 antibody (15061-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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DHRS7 Polyclonal antibody

DHRS7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15061-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CGI 86, CGI-86, DHRS7A, EC:1.1.1.-, Protein SDR34C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15061-1-AP targets DHRS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS7 fusion protein Ag7064 相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 7
Calculated molecular weight 38 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC000637
Gene Symbol DHRS7
Gene ID (NCBI) 51635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHRS7 (Dehydrogenase/Reductase (SDR family) member 7), also known as SDR34C1, retSDR4, is a member of a large family of short-chain dehydrogenases/reductases (SDR). The DHRS7 gene codes for two subtypes and is found on chromosome 14. Isotype 1 (38 kDa) has 339 amino acids, while isotype 2 (32 kDa) contains 289 amino acids (PMID: 26466768). DHRS7 has been demonstrated to be an integral protein facing the lumen of the endoplasmic reticulum with lack of posttranscriptional glycosylation modification(PMID: 24246760).

Protocols

Product Specific Protocols
WB protocol for DHRS7 antibody 15061-1-APDownload protocol
IHC protocol for DHRS7 antibody 15061-1-APDownload protocol
IF protocol for DHRS7 antibody 15061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols