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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHRS7C Polyclonal antibody, PBS Only

DHRS7C Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 26020-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS7C Polyclonal antibody (26020-1-AP)

    WB analysis using 26020-1-AP (same clone as 26020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26020-1-AP (DHRS7C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DHRS7C Polyclonal antibody (26020-1-AP)

    IHC staining of mouse skeletal muscle using 26020-1-AP (same clone as 26020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26020-1-AP (DHRS7C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DHRS7C Polyclonal antibody (26020-1-AP)

    IHC staining of mouse heart using 26020-1-AP (same clone as 26020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26020-1-AP (DHRS7C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26020-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26020-1-PBS targets DHRS7C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS7C fusion protein Ag23392 相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 7C
Observed molecular weight 35 kDa
GenBank accession numberBC147025
Gene symbol DHRS7C
Gene ID (NCBI) 201140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DHRS7C also known as SDR32C2, is a member of the large short-chain dehydrogenase/reductase (SDR) family of enzymes that metabolize steroid hormones, prostaglandins, retinoids, lipids, and xenobiotics (PMID: 19027726). DHRS7C is involved in regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum. DHRS7C is localized to the endo/sarcoplasmic reticulumis, and expression levels were highest in heart and skeletal muscle followed by skin but were not detectable in other organs (PMID: 22143674).