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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHRS9 Polyclonal antibody

DHRS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14560-1-AP

Synonyms

EC:1.1.1.209, EC:1.1.1.105, 3-alpha-HSD, 3-alpha hydroxysteroid dehydrogenase, 3ALPHA HSD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS9 Polyclonal antibody (14560-1-AP)

    WB analysis using 14560-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHRS9 Polyclonal antibody (14560-1-AP)

    WB analysis of HeLa using 14560-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse trachea tissue using DHRS9 Polyclonal antibody (14560-1-AP)

    WB analysis of mouse trachea using 14560-1-AP

    mouse trachea tissue were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DHRS9 Polyclonal antibody (14560-1-AP)

    IHC staining of human lung cancer using 14560-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DHRS9 Polyclonal antibody (14560-1-AP)

    IHC staining of human lung cancer using 14560-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DHRS9 Polyclonal antibody (14560-1-AP)

    IF Staining of A549 using 14560-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using DHRS9 antibody (14560-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DHRS9 Polyclonal antibody (14560-1-AP)

    IF Staining of A549 using 14560-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14560-1-AP (DHRS9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse trachea tissue, HT-29 cells, THP-1 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14560-1-AP targets DHRS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS9 fusion protein Ag6116 相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 9
Calculated molecular weight 35 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC058883
Gene symbol DHRS9
Gene ID (NCBI) 10170
RRIDAB_2277277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dehydrogenase/reductase 9(DHRS9), is a member of the short-chain dehydrogenases/reductases (SDR) family. DHRS9 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. DHRS9 has 4 isoforms with molecular weight of 19, 30, 35, 42 kDa, and demonstrates oxidoreductase activity toward hydroxysteroids. DHRS9 is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus.

Protocols

Product Specific Protocols
WB protocol for DHRS9 antibody 14560-1-APDownload protocol
IHC protocol for DHRS9 antibody 14560-1-APDownload protocol
IF protocol for DHRS9 antibody 14560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype.

Authors - Shuang Bai
View Article
human,mouseIF,WB

EPMA J

Identifying potential signatures for atherosclerosis in the context of predictive, preventive, and personalized medicine using integrative bioinformatics approaches and machine-learning strategies

Authors - Jinling Xu
View Article