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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHTKD1 Polyclonal antibody

DHTKD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27493-1-AP

Synonyms

DHTKD1, KIAA1630

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DHTKD1 Polyclonal antibody (27493-1-AP)

    WB analysis using 27493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27493-1-AP (DHTKD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DHTKD1 Polyclonal antibody (27493-1-AP)

    WB analysis using 27493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27493-1-AP (DHTKD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-13 cells using DHTKD1 Polyclonal antibody (27493-1-AP)

    WB analysis of PC-13 using 27493-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 27493-1-AP (DHTKD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DHTKD1 Polyclonal antibody (27493-1-AP)

    IHC staining of human lung cancer using 27493-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27493-1-AP (DHTKD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DHTKD1 Polyclonal antibody (27493-1-AP)

    IHC staining of human lung cancer using 27493-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27493-1-AP (DHTKD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DHTKD1 Polyclonal antibody (27493-1-AP)

    IF Staining of MCF-7 using 27493-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DHTKD1 antibody (27493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, PC-13 cells, L02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27493-1-AP targets DHTKD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHTKD1 fusion protein Ag26515 相同性解析による交差性が予測される生物種
Full Name dehydrogenase E1 and transketolase domain containing 1
Calculated molecular weight 103 kDa
Observed molecular weight 103 kDa
GenBank accession numberBC007955
Gene symbol DHTKD1
Gene ID (NCBI) 55526
RRIDAB_2880888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DHTKD1 antibody 27493-1-APDownload protocol
IHC protocol for DHTKD1 antibody 27493-1-APDownload protocol
IF protocol for DHTKD1 antibody 27493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Endocrinol (Lausanne)

Knock-Out of DHTKD1 Alters Mitochondrial Respiration and Function, and May Represent a Novel Pathway in Cardiometabolic Disease Risk.

Authors - Chuan Wang
View Article
mouseWB,IHC

J Hepatocell Carcinoma

Liver Regeneration-Related Genes of Nontumor Liver Tissues Predict the Prognosis of Patients with Hepatocellular Carcinoma

Authors - Yanchao Xu
View Article