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  • Featured Product
  • KD/KO Validated

DHX15 Recombinant antibody

DHX15 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F13

Cat no : 82137-1-RR

Synonyms

EC:3.6.4.13, DDX15, DBP1, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DHX15 Recombinant antibody (82137-1-RR)

    WB analysis of HeLa using 82137-1-RR

    WB result of DHX15 antibody (82137-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DHX15 Recombinant antibody (82137-1-RR)

    WB analysis using 82137-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82137-1-RR (DHX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using DHX15 Recombinant antibody (82137-1-RR)

    IP experiment of NIH/3T3 using 82137-1-RR

    IP result of anti-DHX15 (IP:82137-1-RR, 4ug; Detection:82137-1-RR 1:8000) with NIH/3T3 cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DHX15 Recombinant antibody (82137-1-RR)

    IHC staining of mouse testis using 82137-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DHX15 Recombinant antibody (82137-1-RR)

    IHC staining of mouse testis using 82137-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHX15 Recombinant antibody (82137-1-RR)

    IF Staining of HeLa using 82137-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHX15 Recombinant antibody (82137-1-RR)

    IF Staining of HeLa using 82137-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHX15 Recombinant antibody (82137-1-RR)

    IF Staining of HeLa using 82137-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using DHX15 Recombinant antibody (82137-1-RR)

    FC experiment of HeLa using 82137-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DHX15 (82137-1-RR, Clone:1F13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82137-1-RR

    Affinity and Kinetic Characterization of 82137-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inU-251 cells, HeLa cells, U2OS cells, A431 cells, NIH/3T3 cells, C6 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82137-1-RR targets DHX15 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DHX15 fusion protein Ag2895 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 15
Calculated molecular weight 795 aa, 91 kDa
Observed molecular weight90-95 kDa
GenBank accession numberBC035974
Gene symbol DHX15
Gene ID (NCBI) 1665
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kDa.

Protocols

Product Specific Protocols
WB protocol for DHX15 antibody 82137-1-RRDownload protocol
IHC protocol for DHX15 antibody 82137-1-RRDownload protocol
IF protocol for DHX15 antibody 82137-1-RRDownload protocol
IP protocol for DHX15 antibody 82137-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (08-12-2024)

Excellent and sensitive antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: mESCs
DHX15 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:82137-1-RR)