• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHX16 Polyclonal antibody

DHX16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Neutralization, ELISA

Conjugate

Unconjugated

Cat no : 11021-1-AP

Synonyms

EC:3.6.4.13, DEAH-box protein 16, DEAH box protein 16, DDX16, DBP2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of Jurkat using 11021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of HeLa using 11021-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of human brain using 11021-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of mouse testis using 11021-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DHX16 Polyclonal antibody (11021-1-AP)

    IHC staining of human lymphoma using 11021-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11021-1-AP (DHX16 antibody) at dilution of 1:10 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DHX16 Polyclonal antibody (11021-1-AP)

    IF Staining of MCF-7 using 11021-1-AP

    Immunofluorescent analysis of MCF-7 cells, using DHX16 antibody 11021-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, human brain tissue, mouse testis tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11021-1-AP targets DHX16 in WB, IHC, IF/ICC, Neutralization, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX16 fusion protein Ag1482 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 16
Calculated molecular weight 119 kDa
Observed molecular weight 119 kDa
GenBank accession numberBC009392
Gene symbol DHX16
Gene ID (NCBI) 8449
RRIDAB_2245938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DHX16 antibody 11021-1-APDownload protocol
IHC protocol for DHX16 antibody 11021-1-APDownload protocol
IF protocol for DHX16 antibody 11021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanNeutralization

Nat Commun

Molecular basis for the activation of human spliceosome

Authors - Xiechao Zhan
View Article