• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

DHX32 Polyclonal antibody

DHX32 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19808-1-AP

Synonyms

DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, HuDDX32

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using DHX32 Polyclonal antibody (19808-1-AP)

    WB analysis of mouse kidney using 19808-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using DHX32 Polyclonal antibody (19808-1-AP)

    WB analysis of mouse colon using 19808-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using DHX32 Polyclonal antibody (19808-1-AP)

    WB analysis of A431 using 19808-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using DHX32 Polyclonal antibody (19808-1-AP)

    WB analysis of COLO 320 using 19808-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using DHX32 Polyclonal antibody (19808-1-AP)

    IHC staining of human testis using 19808-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DHX32 Polyclonal antibody (19808-1-AP)

    IHC staining of human testis using 19808-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DHX32 Polyclonal antibody (19808-1-AP)

    IHC staining of human kidney using 19808-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DHX32 Polyclonal antibody (19808-1-AP)

    IHC staining of human kidney using 19808-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 40x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inmouse kidney tissue, mouse colon tissue, A431 cells, COLO 320 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19808-1-AP targets DHX32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX32 fusion protein Ag13834 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 32
Calculated molecular weight 743 aa, 84 kDa
Observed molecular weight 84 kDa, 75 kDa
GenBank accession numberBC002473
Gene symbol DHX32
Gene ID (NCBI) 55760
RRIDAB_10641031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicase with an activation-dependent paten of expression and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family are highly conserved enzymes that are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer. This is a rabbit polyclonal anti-DHX32 antibody raised against part of the C-terminal chain of human DHX32.

Protocols

Product Specific Protocols
WB protocol for DHX32 antibody 19808-1-APDownload protocol
IHC protocol for DHX32 antibody 19808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncol Lett

DHX32 expression is an indicator of poor breast cancer prognosis.

Authors - Meng Wang
View Article
humanWB,IHC

Transl Cancer Res

Silencing of DHX32 increases the proliferation of liver cancer cells.

Authors - Min-Jing Cai
  • KD Validated
View Article