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DHX32 Monoclonal antibody

DHX32 Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A4G9

Cat no : 68306-1-Ig

Synonyms

HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHX32 Monoclonal antibody (68306-1-Ig)

    WB analysis using 68306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DHX32 Monoclonal antibody (68306-1-Ig)

    WB analysis of A549 using 68306-1-Ig

    WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using DHX32 Monoclonal antibody (68306-1-Ig)

    WB analysis using 68306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using DHX32 Monoclonal antibody (68306-1-Ig)

    WB analysis of U2OS using 68306-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DHX32 Monoclonal antibody (68306-1-Ig)

    WB analysis using 68306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using DHX32 Monoclonal antibody (68306-1-Ig)

    FC experiment of HeLa using 68306-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug DHX32 Monoclonal antibody (68306-1-Ig, Clone:2A4G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inA431 cells, U2OS cells, rat testis tissue, HeLa cells, A549 cells, mouse testis tissue, MCF-7 cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68306-1-Ig targets DHX32 in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DHX32 fusion protein Ag13956 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 32
Calculated molecular weight 743 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC002473
Gene symbol DHX32
Gene ID (NCBI) 55760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for DHX32 antibody 68306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols