Featured Product
KD/KO Validated

DHX9 Polyclonal antibody

Name: DHX9 Polyclonal antibody
Brand: Proteintech
SKU: 17721-1-AP
Rating: 5.00 (1 reviews)

DHX9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 17721-1-AP

Synonyms

NDH II, LKP, EC:3.6.4.13, DExH-box helicase 9, DDX9

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 17721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 17721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 17721-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 17721-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 17721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
IF Staining of HepG2 using 17721-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 17721-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, rat spleen tissue, rat testis tissue, Jurkat cells
Positive IHC detected in	mouse brain tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 33 publications below
IHC	See 4 publications below
IF	See 6 publications below
IP	See 3 publications below
ELISA	See 1 publications below
CoIP	See 3 publications below
ChIP	See 1 publications below
RIP	See 4 publications below

Product Information

17721-1-AP targets DHX9 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DHX9 fusion protein Ag12104 相同性解析による交差性が予測される生物種
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight	1270 aa, 141 kDa
Observed molecular weight	140 kDa
GenBank accession number	BC014246
Gene symbol	DHX9
Gene ID (NCBI)	1660
RRID	AB_2092506
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Protocols

Product Specific Protocols
WB protocol for DHX9 antibody 17721-1-AP	Download protocol
IHC protocol for DHX9 antibody 17721-1-AP	Download protocol
IF protocol for DHX9 antibody 17721-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Hepatology Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma. Authors - Zhixiang Hu View Article
mouse	WB	Sci Adv R-loops coordinate with SOX2 in regulating reprogramming to pluripotency. Authors - Yaoyi Li View Article
human	RIP	Nat Commun Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1. Authors - Hamed Kooshapur View Article
human	WB	Nat Commun Interaction of 7SK with the Smn complex modulates snRNP production. Authors - Changhe Ji View Article
human	WB	Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Authors - Roy Matkovic View Article
human	WB	Nat Commun PMID: 31792210 Authors - Sunwang Xu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Roy (Verified Customer) (06-12-2024)

Strong DHX9 band detected at the right size by WB (1/1000- 1h incubation at RT) with HeLa extracts.

Applications: Western Blot
Primary Antibody Dilution: 1/1000
Cell Tissue Type: HeLa

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHX9 Polyclonal antibody

DHX9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 17721-1-AP

WB analysis using 17721-1-AP

WB analysis using 17721-1-AP

WB analysis of rat spleen using 17721-1-AP

IHC staining of mouse testis using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse brain using 17721-1-AP

IHC staining of mouse testis using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

IF Staining of HepG2 using 17721-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.

R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.

Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.

Interaction of 7SK with the Smn complex modulates snRNP production.

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

PMID: 31792210

Reviews