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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

    WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DHX9 Monoclonal antibody (67153-1-Ig)

    IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

    IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    IF Staining of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50161-1

    Cytometric bead array standard curve of MP50161-1

    Cytometric bead array standard curve of MP50161-1, DHX9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67153-2-PBS. Detection antibody: 67153-1-PBS. Standard:Ag12104. Range: 1.563-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

DHX9 Monoclonal antibody, PBS Only (Detector)

DHX9 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 67153-1-PBS
Clone No.1B12C10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Synonyms

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67153-1-PBS targets DHX9 as part of a matched antibody pair:

MP50161-1: 67153-2-PBS capture and 67153-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHX9 fusion protein Ag12104 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight 1270 aa, 141 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC014246
Gene Symbol DHX9
Gene ID (NCBI) 1660
RRIDAB_2882450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08211
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].