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mDia1 Polyclonal antibody

Name: mDia1 Polyclonal antibody
Brand: Proteintech
SKU: 20624-1-AP
Rating: 5.00 (1 reviews)

mDia1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 20624-1-AP

Synonyms

DIAPH1, Diaphanous-related formin-1, Diaphanous related formin 1, DIAPH 1, DIAP1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 20624-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 20624-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 20624-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 20624-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 20624-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20624-1-AP
IP result of anti-mDia1 (IP:20624-1-AP, 4ug; Detection:20624-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of mouse kidney using 20624-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20624-1-AP (mDia1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20624-1-AP
Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 20624-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (20624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 20624-1-AP
Immunofluorescent analysis of Hela cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HeLa using 20624-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human mDia1 (20624-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 20624-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, human skeletal muscle tissue, COS-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HUVEC cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 17 publications below
IHC	See 3 publications below
IF	See 9 publications below
ChIP	See 1 publications below

Product Information

20624-1-AP targets mDia1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	mDia1 fusion protein Ag14523 相同性解析による交差性が予測される生物種
Full Name	diaphanous homolog 1 (Drosophila)
Calculated molecular weight	1272 aa, 141 kDa
Observed molecular weight	140-150 kDa, 70 kDa
GenBank accession number	BC007411
Gene symbol	mDia1
Gene ID (NCBI)	1729
RRID	AB_10858618
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

Protocols

Product Specific Protocols
WB protocol for mDia1 antibody 20624-1-AP	Download protocol
IHC protocol for mDia1 antibody 20624-1-AP	Download protocol
IF protocol for mDia1 antibody 20624-1-AP	Download protocol
IP protocol for mDia1 antibody 20624-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC,IF	Circ Res Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways. Authors - Touré Fatouma F View Article
mouse	WB	Dev Cell Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair. Authors - Fernando R Valencia KD Validated View Article
human	WB,IHC	J Cell Biol Dia1 coordinates differentiation and cell sorting in a stratified epithelium. Authors - Robert M Harmon View Article
human	WB, IF	Mol Biol Cell Small molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy. Authors - Jessica D Arden View Article
human	WB	Cells The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells. Authors - Kathryn N Becker View Article
human	WB	PLoS One A Novel Role for p115RhoGEF in Regulation of Epithelial Plasticity. Authors - Swapnil S Kher KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Sammy (Verified Customer) (11-20-2023)

Detects a band around 150kDa.

Applications: Western Blot
Primary Antibody Dilution: 1 in 1000
Cell Tissue Type: HUVEC

mDia1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:20624-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

mDia1 Polyclonal antibody

mDia1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 20624-1-AP

WB analysis of human heart using 20624-1-AP

WB analysis of human skeletal muscle using 20624-1-AP

WB analysis of COS-7 using 20624-1-AP

WB analysis of NIH/3T3 using 20624-1-AP

IP experiment of HeLa using 20624-1-AP

IHC staining of mouse kidney using 20624-1-AP

IF Staining of HepG2 using 20624-1-AP

IF Staining of HeLa using 20624-1-AP

IF Staining of HeLa using 20624-1-AP

FC experiment of HeLa using 20624-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.

Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.

Dia1 coordinates differentiation and cell sorting in a stratified epithelium.

Small molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

A Novel Role for p115RhoGEF in Regulation of Epithelial Plasticity.

Reviews