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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DIDO1 Polyclonal antibody

DIDO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10183-1-AP

Synonyms

death inducer obliterator 1, DATF-1, DATF1, DATF 1, C20orf158

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using DIDO1 Polyclonal antibody (10183-1-AP)

    WB analysis of Raji using 10183-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10183-1-AP (DIDO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DIDO1 Polyclonal antibody (10183-1-AP)

    IHC staining of human breast cancer using 10183-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DIDO1 Polyclonal antibody (10183-1-AP)

    IHC staining of human breast cancer using 10183-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DIDO1 Polyclonal antibody (10183-1-AP)

    IF Staining of HepG2 using 10183-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DIDO1 antibody (10183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DIDO1 Polyclonal antibody (10183-1-AP)

    IF Staining of HepG2 using 10183-1-AP

    Immunofluorescent analysis of HepG2 cells, using DIDO1 antibody 10183-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10183-1-AP targets DIDO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIDO1 fusion protein Ag0237 相同性解析による交差性が予測される生物種
Full Name death inducer-obliterator 1
Calculated molecular weight 59 kDa
Observed molecular weight 300-350 kDa
GenBank accession numberBC000770
Gene symbol DIDO1
Gene ID (NCBI) 11083
RRIDAB_2277375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis.

Protocols

Product Specific Protocols
WB protocol for DIDO1 antibody 10183-1-APDownload protocol
IHC protocol for DIDO1 antibody 10183-1-APDownload protocol
IF protocol for DIDO1 antibody 10183-1-APDownload protocol
FC protocol for DIDO1 antibody 10183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols