DIO2 Polyclonal antibody
DIO2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26513-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, MCF-7 cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 26513-1-AP is DIO2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DIO2 fusion protein Ag24119 相同性解析による交差性が予測される生物種 |
| Full Name | deiodinase, iodothyronine, type II |
| Calculated molecular weight | 273 aa, 31 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC074882 |
| Gene symbol | DIO2 |
| Gene ID (NCBI) | 1734 |
| RRID | AB_2880538 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DIO2 antibody 26513-1-AP | Download protocol |
| IHC protocol for DIO2 antibody 26513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Tumor Cell "Slimming" Regulates Tumor Progression through PLCL1/UCP1-Mediated Lipid Browning. | ||
Nat Chem Biol Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. | ||
Diabetes Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning. | ||
Clin Transl Med NNT-induced tumor cell "slimming" reverses the pro-carcinogenesis effect of HIF2a in tumors. | ||
FASEB J The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting. | ||
Ecotoxicol Environ Saf Exposure to melamine cyanuric acid in adult mice induced thyroid dysfunction and circadian rhythm disorder. |
