Validation Data Gallery
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Tested Applications
| Positive WB detected in | T-47D cells, LNCaP cells, MCF-7 cells |
| Positive IHC detected in | human thyroid cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
| RIP | See 1 publications below |
Product Information
66813-1-Ig targets DIO2 in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DIO2 fusion protein Ag24057 相同性解析による交差性が予測される生物種 |
| Full Name | deiodinase, iodothyronine, type II |
| Calculated molecular weight | 273 aa, 31 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC074882 |
| Gene Symbol | DIO2 |
| Gene ID (NCBI) | 1734 |
| RRID | AB_2882156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DIO2 antibody 66813-1-Ig | Download protocol |
| IHC protocol for DIO2 antibody 66813-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Defective autophagy contributes to endometrial epithelial-mesenchymal transition in intrauterine adhesions.
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Biochem Biophys Rep A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes | ||
Biochem Pharmacol Dysregulated lncSNHG12 suppresses the invasion and migration of trophoblasts by regulating Dio2/Snail axis to involve in recurrent spontaneous abortion
| ||
Thyroid Increased thyroid hormone action alleviates hippocampal damage by down-regulating neuronal type-Ⅰ interferon signaling/necroptosis in diabetes-associated cognitive dysfunction |